ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (3): 497-502.doi: 10.11843/j.issn.0366-6964.2015.03.021

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POMC Expression and Localization Analysis in Different Coat Color Alpaca Skin

WANG Hai-dong1,MA Shu-hui1,LI Ya-nan1,HE Xiao-yan1,YANG Lei2,DONG Chang-sheng1*   

  1. (1.College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China;2.College of Information,Shanxi Agricultural University,Taigu 030801,China)
  • Received:2014-07-28 Online:2015-03-23 Published:2015-03-23

Abstract:

As a color-related gene,POMC plays an crucial role in the formation,development,pigmentation and other aspects of melanocytes.To study the expression level of POMC mRNA and localization of POMC protein in alpaca skin with different coat colors,quantitative real-time PCR (qRT-PCR) and immunohistochemistry were performed.The results showed that the quantity expression of POMC mRNA in brown alpaca was 23 times than in white alpaca (P<0.01),and POMC were expressed in root sheath and epidermis in both brown and white alpaca skin,moreover,the average protein expression of POMC in brown alpaca skin was higher than in white alpaca skin on the average optical density (P<0.05).So POMC may be involved in the coat color formation.

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